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MPAPASS software enables stitched multiplex, multidimensional EV repertoire analysis and a standard framework for reporting bead-based assays.


ABSTRACT: Extracellular vesicles (EVs) of various types are released or shed from all cells. EVs carry proteins and contain additional protein and nucleic acid cargo that relates to their biogenesis and cell of origin. EV cargo in liquid biopsies is of widespread interest owing to its ability to provide a retrospective snapshot of cell state at the time of EV release. For the purposes of EV cargo analysis and repertoire profiling, multiplex assays are an essential tool in multiparametric analyte studies but are still being developed for high-parameter EV protein detection. Although bead-based EV multiplex analyses offer EV profiling capabilities with conventional flow cytometers, the utilization of EV multiplex assays has been limited by the lack of software analysis tools for such assays. To facilitate robust EV repertoire studies, we developed multiplex analysis post-acquisition analysis (MPAPASS) open-source software for stitched multiplex analysis, EV database-compatible reporting, and visualization of EV repertoires.

SUBMITTER: Welsh JA 

PROVIDER: S-EPMC9017130 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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MPA<sub>PASS</sub> software enables stitched multiplex, multidimensional EV repertoire analysis and a standard framework for reporting bead-based assays.

Welsh Joshua A JA   Killingsworth Bryce B   Kepley Julia J   Traynor Tim T   Cook Sean S   Savage Jason J   Marte Jenn J   Lee Min-Jung MJ   Maeng Hoyoung M HM   Pleet Michelle L ML   Magana Setty S   Gorgens André A   Maire Cecile L CL   Lamszus Katrin K   Ricklefs Franz L FL   Merino Maria J MJ   Linehan W Marston WM   Greten Tim T   Cooks Tomer T   Harris Curtis C CC   Apolo Andrea A   Abdel-Mageed Asim A   Ivanov Alexander R AR   Trepel Jane B JB   Roth Matthew M   Tkach Mercedes M   Milosavljevic Aleksandar A   Théry Clotilde C   LeBlanc Amy A   Berzofsky Jay A JA   Ruppin Eytan E   Aldape Kenneth K   Camphausen Kevin K   Gulley James L JL   Ghiran Ionita I   Jacobson Steve S   Jones Jennifer C JC  

Cell reports methods 20220124 1


Extracellular vesicles (EVs) of various types are released or shed from all cells. EVs carry proteins and contain additional protein and nucleic acid cargo that relates to their biogenesis and cell of origin. EV cargo in liquid biopsies is of widespread interest owing to its ability to provide a retrospective snapshot of cell state at the time of EV release. For the purposes of EV cargo analysis and repertoire profiling, multiplex assays are an essential tool in multiparametric analyte studies b  ...[more]

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