A comprehensive approach for genome-wide efficiency profiling of DNA modifying enzymes.

ABSTRACT: A precise understanding of DNA methylation dynamics is of great importance for a variety of biological processes including cellular reprogramming and differentiation. To date, complex integration of multiple and distinct genome-wide datasets is required to realize this task. We present GwEEP (genome-wide epigenetic efficiency profiling) a versatile approach to infer dynamic efficiencies of DNA modifying enzymes. GwEEP relies on genome-wide hairpin datasets, which are translated by a hidden Markov model into quantitative enzyme efficiencies with reported confidence around the estimates. GwEEP predicts de novo and maintenance methylation efficiencies of Dnmts and furthermore the hydroxylation efficiency of Tets. Its design also allows capturing further oxidation processes given availa

SUBMITTER: Kyriakopoulos C 

PROVIDER: S-EPMC9017147 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature