A Proteomic Landscape of Candida albicans in the Stepwise Evolution to Fluconazole Resistance.

ABSTRACT: As an opportunistic fungal pathogen, Candida albicans is a major cause of superficial and systemic infections in immunocompromised patients. The increasing rate of azole resistance in C. albicans has brought further challenges to clinical therapy. In this study, we collected five isogenic C. albicans strains recovered over discrete intervals from an HIV-infected patient who suffered 2-year recurrent oropharyngeal candidiasis. Azole resistance was known from the clinical history to have developed gradually in this patient, and this was confirmed by MIC assays of each strain. Proteomic techniques can be used to investigate more comprehensively how resistance develops in pathogenic fungi over time. Our study is the first to use tandem mass tag (TMT) labeling combined with liquid chromatograph

SUBMITTER: Song N 

PROVIDER: S-EPMC9017357 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature