Cell-free DNA as a biomarker after lung transplantation: A proof-of-concept study.

ABSTRACT:

Background

Lung transplantation (LTx) is a lifesaving procedure burdened with limited long-term survival. The most common cause of death after LTx is chronic lung allograft dysfunction (CLAD). Today, useful biomarkers for the detection of CLAD are lacking. Circulating cell-free DNA (cfDNA) is released during cellular decay and can be detected using polymerase chain reaction (PCR). Thus, donor-derived cfDNA in recipient serum indicates cellular decay in the transplanted organ. In the current study, we explore the possibility of using a novel PCR method to detect cfDNA as a biomarker for clinical events, especially CLAD.

Methods

Four patients were retrospectively tested for levels of both donor and recipient-derived cfDNA using digital droplet PCR after targeted preamplificati

SUBMITTER: Magnusson JM 

PROVIDER: S-EPMC9017613 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature