LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters.

ABSTRACT: Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional luminometer instruments and the versatility of their use in different experimental conditions. Therefore, we aimed to develop a small, affordable luminometer allowing continuous measurement of luciferase activity, designed for inclusion into various kinds of tissue culture incubators. Here, we introduce LuminoCell-an open-source platform for the construction of an affordable, sensitive, and portable luminometer capable of real-time monitoring in-cell luciferase activity. The LuminoCell costs $40, requires less than 1 h to assemble, and it is capable of perf

SUBMITTER: Weissova K 

PROVIDER: S-EPMC9018015 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature