Antiretroviral drug exposure in lymph nodes is heterogeneous and drug dependent.

ABSTRACT:

Introduction

HIV reservoirs and infected cells may persist in tissues with low concentrations of antiretrovirals (ARVs). Traditional pharmacology methods cannot assess variability in ARV concentrations within morphologically complex tissues, such as lymph nodes (LNs). We evaluated the distribution of six ARVs into LNs and the proximity of these ARVs to CD4⁺ T cells and cell-associated RT-SHIV viral RNA.

Methods

Between December 2014 and April 2017, RT-SHIV infected (SHIV+; N = 6) and healthy (SHIV-; N = 6) male rhesus macaques received two selected four-drug combinations of six ARVs over 10 days to attain steady-state conditions. Serial cryosections of axillary LN were analysed by a multimodal imaging approach that combined mass spectrometry imaging (MSI) for ARV

SUBMITTER: Rosen EP 

PROVIDER: S-EPMC9018350 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature