A protocol to assess cellular bioenergetics in flavivirus-infected cells.
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ABSTRACT:
SUBMITTER: Low JZH
PROVIDER: S-EPMC9018450 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Publications
A protocol to assess cellular bioenergetics in flavivirus-infected cells.
Low John Z H JZH Yau Clement C Zhang Summer L X SLX Tan Hwee Cheng HC Ooi Eng Eong EE Chan Kuan Rong KR
STAR protocols 20220411 2
Aberrant cellular bioenergetics has detrimental consequences in host cells. For instance, pathogenic Zika virus strains can suppress mitochondria respiration and glycolytic functions, disrupting cellular bioenergetics that leads to apoptosis. Herein, we describe methods for flavivirus propagation, titering and infection, cell preparation, and procedures for mitochondrial and glycolytic stress tests. The protocol enables assessment of cellular respiration and glycolytic flux in flavivirus-infecte ...[more]
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2018-11-06 | GSE122121 | GEO