In situ generation of a Zwitterionic fluorescent probe for detection of human serum albumin protein.

ABSTRACT: In this article, a new approach for human serum albumin selective fluorophore design has been reported. The fluorophore reported here comprises a substituted phenol donor and a cationic benzo[e]indolium acceptor connected with a π bond. Originally, the cationic fluorophore did not bind with human serum albumin. Upon deprotonation of the phenolic-OH by a water molecule the cationic form was transformed into an active zwitterionic form. Spectroscopic studies and theoretical calculations revealed that the new active form remained in a zwitterionic state in neutral aqueous solution, and it formed a strong supramolecular complex with human serum albumin. The spontaneous complexation resulted multi-fold increase of fluorescence intensity which increased linearly with the concentrations of the pr

SUBMITTER: Choudhury R 

PROVIDER: S-EPMC9018593 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature