Sequence Conservation Does Not Always Signify a Functional Imperative as Observed in the Nitroreductase Superfamily.

ABSTRACT: Consensus sequences have the potential to help classify the structure and function of proteins and highlight key regions that may contribute to their biological properties. Often, the level of significance will track with the extent of sequence conservation, but this should not be considered universal. Arg and Lys dominate a position adjacent to the N1 and C2 carbonyl of flavin mononucleotide (FMN) bound in the proteins of the nitroreductase superfamily. Although this placement satisfies expectations for stabilizing the reduced form of FMN, the substitution of these residues in three subfamilies promoting distinct reactions demonstrates their importance to catalysis as only modest. Replacing Arg34 with Lys, Gln, or Glu enhances FMN binding to a flavin destructase (BluB) by twofold and dimi

SUBMITTER: Musila JM 

PROVIDER: S-EPMC9018611 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature