Establishment of a Transient and Stable Transfection System for Babesia duncani Using a Homologous Recombination Strategy.

ABSTRACT: Genetic modification provides an invaluable molecular tool to dissect the biology and pathogenesis of pathogens. However, no report is available about the genetic modification of Babesia duncani, a pathogen responsible for human babesiosis that is widespread in North America, suggesting the necessity to develop a genetic manipulation method to improve the strategies for studying and understanding the biology of protozoan pathogens. The establishment of a genetic modification method requires promoters, selectable markers, and reporter genes. Here, the double-copy gene elongation factor-1α (ef-1α) and its promoters were amplified by conventional PCR and confirmed by sequencing. We established a transient transfection system by using the ef-1αB promoter and the rep

SUBMITTER: Wang S 

PROVIDER: S-EPMC9019647 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature