Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses.

ABSTRACT: Microglia and astrocytes are implicated in aging and age-related diseases. Here, we present a protocol to isolate and culture these glia cells from the murine brain. The protocol consists of two parts: magnetic sorting of adult microglia and mechanical/magnetic sorting of adult microglia and astrocytes. We then describe the characterization of these glial cells by flow cytometry and immunohistochemistry. Microglia isolated from aged mice maintain age-related phenotype during culture. These purified glia cells can be applied in ex vivo studies.

SUBMITTER: Milner MT 

PROVIDER: S-EPMC9019703 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature