Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains.

ABSTRACT:

Background

Microbial lipids are found to be an interesting green alternative to expand available oil sources for the chemical industry. Yeasts are considered a promising platform for sustainable lipid production. Remarkably, some oleaginous yeasts have even shown the ability to grow and accumulate lipids using unusual carbon sources derived from organic wastes, such as volatile fatty acids. Recent research efforts have been focused on developing rapid and accurate fluorometric methods for the quantification of intracellular yeast lipids. Nevertheless, the current methods are often tedious and/or exhibit low reproducibility.

Results

This work evaluated the reliability of different fluorescence measurements (fluorescence intensity, total area and fluorescence quantum yield) us

SUBMITTER: Morales-Palomo S 

PROVIDER: S-EPMC9019942 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature