Organellar microcapture to extract nuclear and plastid DNA from recalcitrant wood specimens and trace evidence.

ABSTRACT:

Background

Illegal logging is a global crisis with significant environmental, economic, and social consequences. Efforts to combat it call for forensic methods to determine species identity, provenance, and individual identification of wood specimens throughout the forest products supply chain. DNA-based methodologies are the only tools with the potential to answer all three questions and the only ones that can be calibrated "non-destructively" by using leaves or other plant tissue and take advantage of publicly available DNA sequence databases. Despite the potential that DNA-based methods represent for wood forensics, low DNA yield from wood remains a limiting factor because, when compared to other plant tissues, wood has few living DNA-containing cells at functional maturity, it

SUBMITTER: Costa A 

PROVIDER: S-EPMC9019980 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature