Addressing the role of PKD3 in the T cell compartment with knockout mice.

ABSTRACT:

Background

The Protein kinase D3 (PKD3) has been implicated in signal transduction downstream of the T cell receptor (TCR). However, its role for the activation of primary T lymphocytes has not been elucidated so far.

Methods

Expression of PKD isoforms in primary murine T cells was determined by RT-PCR and SDS-Page. A germline PKD3-knockout mouse line was analyzed for its immune response to OVA/alum intraperitoneal immunization. Phenotyping of the T cell compartment ex vivo as well as upon stimulation in vitro was performed by flow cytometry. Additionally, cytokine expression was assessed by flow cytometry, RT-PCR and Luminex technology.

Results

PKD expression in T cells is modulated by TCR stimulation, leading to a rapid down-regulation on mRNA and on protein level.

SUBMITTER: Koutnik J 

PROVIDER: S-EPMC9020081 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature