Dataset Information

Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy.

ABSTRACT:

Background and aims

Analysis of atherosclerotic plaque composition is a vital tool for unraveling the pathological metabolic processes that contribute to plaque growth.

Methods

We visualize the constitution of human carotid plaques by mid-infrared optoacoustic microscopy (MiROM), a method for label-free analytic histology that requires minimal tissue preparation, rapidly yielding large field-of-view en-face images with a resolution of a few micrometers. We imaged endarterectomy specimens (n = 3, 12 sections total) at specific vibrational modes, targeting carbohydrates, lipids and proteins. Additionally, we recorded spectra at selected tissue locations. We identified correlations in the variability in this high-dimensional data set using non-negative matrix factorization (NMF).

Results

We visualized high-risk plaque features with molecular assignment. Consistent NMF components relate to different dominant tissue constituents, dominated by lipids, proteins, and cholesterol and carbohydrates respectively.

Conclusions

These results introduce MiROM as an innovative, stain-free, analytic histology technology for the biochemical characterization of complex human vascular pathology.

SUBMITTER: Visscher M

PROVIDER: S-EPMC9020099 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Publications

Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy.

Visscher Mirjam M Pleitez Miguel A MA Van Gaalen Kim K Nieuwenhuizen-Bakker Ingeborg M IM Ntziachristos Vasilis V Van Soest Gijs G

Photoacoustics 20220411

<h4>Background and aims</h4>Analysis of atherosclerotic plaque composition is a vital tool for unraveling the pathological metabolic processes that contribute to plaque growth.<h4>Methods</h4>We visualize the constitution of human carotid plaques by mid-infrared optoacoustic microscopy (MiROM), a method for label-free analytic histology that requires minimal tissue preparation, rapidly yielding large field-of-view en-face images with a resolution of a few micrometers. We imaged endarterectomy sp ...[more]

PMID: 35465607

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Dataset Information

Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy.

Background and aims

Methods

Results

Conclusions

Publications

Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy.

Similar Datasets

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