Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy.

ABSTRACT:

Background and aims

Analysis of atherosclerotic plaque composition is a vital tool for unraveling the pathological metabolic processes that contribute to plaque growth.

Methods

We visualize the constitution of human carotid plaques by mid-infrared optoacoustic microscopy (MiROM), a method for label-free analytic histology that requires minimal tissue preparation, rapidly yielding large field-of-view en-face images with a resolution of a few micrometers. We imaged endarterectomy specimens (n = 3, 12 sections total) at specific vibrational modes, targeting carbohydrates, lipids and proteins. Additionally, we recorded spectra at selected tissue locations. We identified correlations in the variability in this high-dimensional data set using non-negative matrix factorization (NMF

SUBMITTER: Visscher M 

PROVIDER: S-EPMC9020099 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature