STL-seq reveals pause-release and termination kinetics for promoter-proximal paused RNA polymerase II transcripts.

ABSTRACT: Despite the critical regulatory function of promoter-proximal pausing, the influence of pausing kinetics on transcriptional control remains an active area of investigation. Here, we present Start-TimeLapse-seq (STL-seq), a method that captures the genome-wide kinetics of short, capped RNA turnover and reveals principles of regulation at the pause site. By measuring the rates of release into elongation and premature termination through the inhibition of pause release, we determine that pause-release rates are highly variable, and most promoter-proximal paused RNA polymerase II molecules prematurely terminate (∼80%). The preferred regulatory mechanism upon a hormonal stimulus (20-hydroxyecdysone) is to influence pause-release rather than termination rates. Transcriptional shutdown occurs con

SUBMITTER: Zimmer JT 

PROVIDER: S-EPMC9020433 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature