Bacterial DNA load in Staphylococcus aureus bacteremia is significantly higher in intravascular infections.

ABSTRACT:

Objectives

Determination of pathogen-specific bacterial DNA load (BDL) in blood has been shown to be directly correlated with severity of infection in patients with bacteremia. In the diagnostic work-up of patients with Staphylococcus aureus bacteremia (SAB), determination of the primary focus is imperative, because of implications for treatment duration, and ultimately prognosis. Here we investigate whether measurement of BDL in patients with SAB can distinguish between intravascular and extravascular foci of infection.

Methods

In a consecutive cohort of 43 patients with positive blood cultures with Staphylococcus aureus, we performed a quantitative PCR on whole blood to detect the bacterial DNA load. Infections were classified into 3 categories: i) soft tissue infections a

SUBMITTER: Nieman AE 

PROVIDER: S-EPMC9020692 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature