Clinical significance and intestinal microbiota composition in immunocompromised children with norovirus gastroenteritis.

ABSTRACT:

Background

Norovirus (NoV) infection is common in pediatric patients with immunodeficiency and is more likely to cause severe disease. Objective Our study aims to figure out the clinical differences and distribution of intestinal microbiota in immunocompromised children with NoV gastroenteritis.

Methods

Pediatric patients admitted to Shang-Ho Hospital with diagnosis of acute gastroenteritis including different immune status were enrolled and their medical records were reviewed. NoV gastroenteritis was validated using RT-PCR molecular methods. Viral shedding period was determined by real-time RT-PCR assays. Intestinal microbiota enrichment analysis was carried out by next generation sequencing after fecal DNA extraction and subsequent Linear Discriminant Analysis (LDA) Effect

SUBMITTER: Lin PC 

PROVIDER: S-EPMC9020708 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature