Target-triggered cascade signal amplification for sensitive electrochemical detection of SARS-CoV-2 with clinical application.

ABSTRACT: The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the outbreak of the 2019 coronavirus (COVID-19) disease, which greatly challenges the global economy and health. Simple and sensitive diagnosis of COVID-19 at the early stage is important to prevent the spread of pandemics. Herein, we have proposed a target-triggered cascade signal amplification in this work for sensitive analysis of SARS-CoV-2 RNA. Specifically, the presence of SARS-CoV-2 RNA can trigger the catalytic hairpin assembly to generate plenty of DNA duplexes with free 3'-OH termini, which can be recognized and catalyzed by the terminal deoxynucleotidyl transferase (TdT) to generate long strand DNA. The prolonged DNA can absorb substantial Ru(NH₃)₆³⁺ molecules v

SUBMITTER: Deng Y 

PROVIDER: S-EPMC9020774 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature