Nonlinear control of transcription through enhancer-promoter interactions.

ABSTRACT: Chromosome structure in mammals is thought to regulate transcription by modulating three-dimensional interactions between enhancers and promoters, notably through CTCF-mediated loops and topologically associating domains (TADs)^1-4. However, how chromosome interactions are actually translated into transcriptional outputs remains unclear. Here, to address this question, we use an assay to position an enhancer at large numbers of densely spaced chromosomal locations relative to a fixed promoter, and measure promoter output and interactions within a genomic region with minimal regulatory and structural complexity. A quantitative analysis of hundreds of cell lines reveals that the transcriptional effect of an enhancer depends on its contact probabilities with the promoter through a n

SUBMITTER: Zuin J 

PROVIDER: S-EPMC9021019 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature