Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells.

ABSTRACT: The interaction between a cell and its environment shapes fundamental intracellular processes such as cellular metabolism. In most cases growth rate is treated as a proximal metric for understanding the cellular metabolic status. However, changes in growth rate might not reflect metabolic variations in individuals responding to environmental fluctuations. Here we use single-cell microfluidics-microscopy combined with transcriptomics, proteomics and mathematical modelling to quantify the accumulation of glucose within Escherichia coli cells. In contrast to the current consensus, we reveal that environmental conditions which are comparatively unfavourable for growth, where both nutrients and salinity are depleted, increase glucose accumulation rates in individual bacteria and population subs

SUBMITTER: Glover G 

PROVIDER: S-EPMC9021252 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature