Tension can directly suppress Aurora B kinase-triggered release of kinetochore-microtubule attachments.

ABSTRACT: Chromosome segregation requires sister kinetochores to attach microtubules emanating from opposite spindle poles. Proper attachments come under tension and are stabilized, but defective attachments lacking tension are released, giving another chance for correct attachments to form. This error correction process depends on Aurora B kinase, which phosphorylates kinetochores to destabilize their microtubule attachments. However, the mechanism by which Aurora B distinguishes tense versus relaxed kinetochores remains unclear because it is difficult to detect kinase-triggered detachment and to manipulate kinetochore tension in vivo. To address these challenges, we apply an optical trapping-based assay using soluble Aurora B and reconstituted kinetochore-microtubule attachments. Strikingly, the t

SUBMITTER: de Regt AK 

PROVIDER: S-EPMC9021268 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature