TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancer.

ABSTRACT: RNA modifications are important regulatory elements of RNA functions. However, most genome-wide mapping of RNA modifications has focused on messenger RNAs and transfer RNAs, but such datasets have been lacking for small RNAs. Here we mapped N¹-methyladenosine (m¹A) in the cellular small RNA space. Benchmarked with synthetic m¹A RNAs, our workflow identified specific groups of m¹A-containing small RNAs, which are otherwise disproportionally under-represented. In particular, 22-nucleotides long 3' tRNA-fragments are highly enriched for TRMT6/61A-dependent m¹A located within the seed region. TRMT6/61A-dependent m¹A negatively affects gene silencing by tRF-3s. In urothelial carcinoma of the bladder, where TRMT6/61A is over-expre

SUBMITTER: Su Z 

PROVIDER: S-EPMC9021294 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature