Time Series Transcriptome Analysis in Medicago truncatula Shoot and Root Tissue During Early Nodulation.

ABSTRACT: In response to colonization by rhizobia bacteria, legumes are able to form nitrogen-fixing nodules in their roots, allowing the plants to grow efficiently in nitrogen-depleted environments. Legumes utilize a complex, long-distance signaling pathway to regulate nodulation that involves signals in both roots and shoots. We measured the transcriptional response to treatment with rhizobia in both the shoots and roots of Medicago truncatula over a 72-h time course. To detect temporal shifts in gene expression, we developed GeneShift, a novel computational statistics and machine learning workflow that addresses the time series replicate the averaging issue for detecting gene expression pattern shifts under different conditions. We identified both known and novel genes that are regulated d

SUBMITTER: Gao Y 

PROVIDER: S-EPMC9021838 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature