A Novel Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Yersinia pestis.

ABSTRACT: Rapid detection of Yersinia pestis, the causative agent of plague, is essential during field investigations to enable prompt control measures for prevention of the spread of the disease. Affordable, efficient, reliable, and simple detection assays are extremely useful, particularly in plague-endemic regions with limited resources. We developed a loop-mediated isothermal amplification (LAMP) assay that detects Y. pestis within 30 min by simply incubating at 65°C on a dry bath heater. The assay targeted the caf1A gene that is situated on the pMT1 plasmid using six specific primers. Y. pestis presence is visually detected based on the color change in the reactions. For comparison of the assay performance, a real-time LAMP with fluorescent dye detection was conducte

SUBMITTER: Bai Y 

PROVIDER: S-EPMC9022072 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature