Therapeutic Antibody Discovery in Infectious Diseases Using Single-Cell Analysis.

ABSTRACT: Since the discovery of mouse hybridoma technology by Kohler and Milstein in 1975, significant progress has been made in monoclonal antibody production. Advances in B cell immortalization and phage display technologies have generated a myriad of valuable monoclonal antibodies for diagnosis and treatment. Technological breakthroughs in various fields of 'omics have shed crucial insights into cellular heterogeneity of a biological system in which the functional individuality of a single cell must be considered. Based on this important concept, remarkable discoveries in single-cell analysis have made in identifying and isolating functional B cells that produce beneficial therapeutic monoclonal antibodies. In this review, we will discuss three traditional methods of antibody discovery. Recent t

SUBMITTER: Voigt A 

PROVIDER: S-EPMC9022094 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature