HiIDDD: a high-throughput imaging pipeline for the quantitative detection of DNA damage in primary human immune cells.

ABSTRACT: DNA damage is a prominent biomarker for numerous diseases, including cancer, as well as for the aging process. Detection of DNA damage routinely relies on traditional microscopy or cytometric methods. However, these techniques are typically of limited throughput and are not ideally suited for large-scale longitudinal and population studies that require analysis of large sample sets. We have developed HiIDDD (High-throughput Immune cell DNA Damage Detection), a robust, quantitative and single-cell assay that measures DNA damage by high-throughput imaging using the two major DNA damage markers 53BP1 and [Formula: see text]-H2AX. We demonstrate sensitive detection with low inter-assay variability of DNA damage in various types of freshly isolated and cryopreserved primary human immune cells,

SUBMITTER: Gallant K 

PROVIDER: S-EPMC9022135 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature