Exhausted phenotype of circulating CD8⁺ T cell subsets in hepatitis B virus carriers.

ABSTRACT:

Background

Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted CD8⁺ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted CD8⁺ T cells in HBV carriers.

Methods

We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of CD8⁺ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription-PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry.

Results

There were no significant differences in the baseline characteristics between the 3

SUBMITTER: Jiang D 

PROVIDER: S-EPMC9022260 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature