Subfamily-specific differential contribution of individual monomers and the tether sequence to mouse L1 promoter activity.

ABSTRACT:

Background

The internal promoter in L1 5'UTR is critical for autonomous L1 transcription and initiating retrotransposition. Unlike the human genome, which features one contemporarily active subfamily, four subfamilies (A_I, Gf_I and Tf_I/II) have been amplifying in the mouse genome in the last one million years. Moreover, mouse L1 5'UTRs are organized into tandem repeats called monomers, which are separated from ORF1 by a tether domain. In this study, we aim to compare promoter activities across young mouse L1 subfamilies and investigate the contribution of individual monomers and the tether sequence.

Results

We observed an inverse relationship between subfamily age and the average number of monomers among evolutionarily young mouse L1 subfamilies. The youngest subgroup (A_I

SUBMITTER: Kong L 

PROVIDER: S-EPMC9022269 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature