Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum.

ABSTRACT: A PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum. One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.

SUBMITTER: Durand R 

PROVIDER: S-EPMC90225 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature