Tle4z1 Facilitate the Male Sexual Differentiation of Chicken Embryos.

ABSTRACT:

Background

Sex differentiation is a complex and precisely regulated process by multiple genes in chicken. However, it is still unclear on the key genes of sex differentiation.

Objective

To explore the function of Tle4z1 screened by RNA-seq sequencing on sex differentiation during the development of chicken embryos.

Methods

Tle4z1 was differentially expressed from the RNA-seq of ESCs and PGCs in male and female chickens. Then, we established an effective method to overexpression or knocking down the expression of Tle4z1 in ovo and in vitro, respectively. Histomorphological observation, qRT-PCR and ELISA were applied to detect the function of Tle4z1 in the process of male sex differentiation by injecting vectors into embryos at day 0.<

SUBMITTER: Chen C 

PROVIDER: S-EPMC9022655 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature