Development and evaluation of ActSeq: A targeted next-generation sequencing panel for clinical oncology use.

ABSTRACT:

Purpose

The demand for high-throughput genetic profiling of somatic mutations in cancer tissues is growing. We sought to establish a targeted next generation sequencing (NGS) panel test for clinical oncology practice.

Methods

Customized probes were designed to capture exonic regions of 141 genes selected for the panel, which was aimed for the detection of clinically actionable genetic variations in cancer, including KRAS, NRAS, BRAF, ALK, ROS1, KIT and EGFR. The size of entire targeted regions is 0.8 Mb. Library preparation used NEBNext Ultra II FS kit coupled with target enrichment. Paired-end sequencing was run on Illumina NextSeq 500 at a read length of 150 nt. A bioinformatics workflow focusing on single nucleotide variant and short insertions and deletions (SNV/indel) d

SUBMITTER: Shi Z 

PROVIDER: S-EPMC9022865 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature