Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion.

ABSTRACT: Up to seven members of the histone H1 family may contribute to chromatin compaction and its regulation in human somatic cells. In breast cancer cells, knock-down of multiple H1 variants deregulates many genes, promotes the appearance of genome-wide accessibility sites and triggers an interferon response via activation of heterochromatic repeats. However, how these changes in the expression profile relate to the re-distribution of H1 variants as well as to genome conformational changes have not been yet studied. Here, we combined ChIP-seq of five endogenous H1 variants with Chromosome Conformation Capture analysis in wild-type and H1.2/H1.4 knock-down T47D cells. The results indicate that H1 variants coexist in the genome in two large groups depending on the local GC content and that their

SUBMITTER: Serna-Pujol N 

PROVIDER: S-EPMC9023279 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature