The DNA polymerase of bacteriophage YerA41 replicates its T-modified DNA in a primer-independent manner.

ABSTRACT: Yersinia phage YerA41 is morphologically similar to jumbo bacteriophages. The isolated genomic material of YerA41 could not be digested by restriction enzymes, and used as a template by conventional DNA polymerases. Nucleoside analysis of the YerA41 genomic material, carried out to find out whether this was due to modified nucleotides, revealed the presence of a ca 1 kDa substitution of thymidine with apparent oligosaccharide character. We identified and purified the phage DNA polymerase (DNAP) that could replicate the YerA41 genomic DNA even without added primers. Cryo-electron microscopy (EM) was used to characterize structural details of the phage particle. The storage capacity of the 131 nm diameter head was calculated to accommodate a significantly longer genome than that of the 145 5

SUBMITTER: Gomez-Raya-Vilanova MV 

PROVIDER: S-EPMC9023294 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature