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Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape.

ABSTRACT: Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency of monoclonal antibodies (mAbs) and identify viral escape mutants to antibody neutralization for severe respiratory syndrome coronavirus 2 (SARS-CoV-2). For complete details on the use and execution of this protocol, please refer to Zost et al. (2020) and Suryadevara et al. (2021).

SUBMITTER: Suryadevara N

PROVIDER: S-EPMC9023333 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape.

Suryadevara Naveenchandra N Gilchuk Pavlo P Zost Seth J SJ Mittal Nikhil N Zhao Li Leyna LL Crowe James E JE Carnahan Robert H RH

STAR protocols 20220422 2

Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency of monoclonal antibodies (mAbs) and identify viral escape mutants to antibody neutralization for severe respiratory syndrome coronavirus 2 (SARS-CoV-2). For complete details on the use and execution of this protocol, please refer to Z ...[more]

PMID: 35578733

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