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Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids.

ABSTRACT: Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).

SUBMITTER: Eriksen AZ

PROVIDER: S-EPMC9023348 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids.

Eriksen Anne Zebitz AZ Møller Rasmus R Makovoz Bar B tenOever Benjamin R BR Blenkinsop Timothy A TA

STAR protocols 20220422 2

Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaq ...[more]

PMID: 35664254

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