Detection of recurrences using serum miR-371a-3p during active surveillance in men with stage I testicular germ cell tumours.

ABSTRACT:

Background

MiR-371a-3p predicts the presence of a macroscopic non-teratomatous germ cell tumour (GCT). We hypothesised that miR-371a-3p can also detect recurrence during active surveillance (AS) of stage I GCT.

Methods

We prospectively collected serum samples of 33 men. Relative expression of serum miR-371a-3p levels was determined at each follow-up visit using real-time quantitative reverse transcription-polymerase chain reaction.

Results

Recurrence was detected using standard follow-up investigations in 10/33 patients (30%) after a median of 7 months. Directly after orchiectomy, miR-371a-3p levels were not elevated in any of the 15 patients with available post-orchiectomy samples. However, all ten recurring patients exhibited increasing miR-371a-3p levels during fol

SUBMITTER: Fankhauser CD 

PROVIDER: S-EPMC9023438 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature