Next generation automated traceless cell chromatography platform for GMP-compliant cell isolation and activation.

ABSTRACT: Large-scale target cell isolation from patient blood preparations is one of the critical operations during drug product manufacturing for personalized cell therapy in immuno-oncology. Use of high-affinity murine antibody coated magnetic nanoparticles that remain on isolated cells is the current standard applied for this purpose. Here, we present the transformation of previously described technology - non-magnetic immunoaffinity column chromatography-based cell selection with reversible reagents into a new clinical-grade cell isolation platform called Automated Traceless Cell affinity chromatography (ATC). ATC is a fully closed and GMP-compliant cell selection and manufacturing system. Reversibility of reagents enables (sequential) positive cell selection, optionally in combination with dep

SUBMITTER: Radisch S 

PROVIDER: S-EPMC9023455 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature