MYB interacts with androgen receptor, sustains its ligand-independent activation and promotes castration resistance in prostate cancer.

ABSTRACT:

Background

Aberrant activation of androgen receptor signalling following castration therapy is a common clinical observation in prostate cancer (PCa). Earlier, we demonstrated the role of MYB overexpression in androgen-depletion resistance and PCa aggressiveness. Here, we investigated MYB-androgen receptor (AR) crosstalk and its functional significance.

Methods

Interaction and co-localization of MYB and AR were examined by co-immunoprecipitation and immunofluorescence analyses, respectively. Protein levels were measured by immunoblot analysis and enzyme-linked immunosorbent assay. The role of MYB in ligand-independent AR transcriptional activity and combinatorial gene regulation was studied by promoter-reporter and chromatin immunoprecipitation assays. The functional signifi

SUBMITTER: Srivastava SK 

PROVIDER: S-EPMC9023474 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature