Colonic Microbial Abundances Predict Adenoma Formers.

ABSTRACT:

Objective

We aimed to examine associations between the oral, fecal, and mucosal microbiome communities and adenoma formation.

Summary background data

Data are limited regarding the relationships between microbiota and preneoplastic colorectal lesions.

Methods

Individuals undergoing screening colonoscopy were prospectively enrolled and divided into adenoma and nonadenoma formers. Oral, fecal, nonadenoma and adenoma-adjacent mucosa were collected along with clinical and dietary information. 16S rRNA gene libraries were generated using V4 primers. DADA2 processed sequence reads and custom R-scripts quantified microbial diversity. Linear regression identified differential taxonomy and diversity in microbial communities and machine learning identified adenoma former microb

SUBMITTER: Watson KM 

PROVIDER: S-EPMC9023594 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature