Integrated profiling of human pancreatic cancer organoids reveals chromatin accessibility features associated with drug sensitivity.

ABSTRACT: Chromatin accessibility plays an essential role in controlling cellular identity and the therapeutic response of human cancers. However, the chromatin accessibility landscape and gene regulatory network of pancreatic cancer are largely uncharacterized. Here, we integrate the chromatin accessibility profiles of 84 pancreatic cancer organoid lines with whole-genome sequencing data, transcriptomic sequencing data and the results of drug sensitivity analysis of 283 epigenetic-related chemicals and 5 chemotherapeutic drugs. We identify distinct transcription factors that distinguish molecular subtypes of pancreatic cancer, predict numerous chromatin accessibility peaks associated with gene regulatory networks, discover regulatory noncoding mutations with potential as cancer drivers, and reveal

SUBMITTER: Shi X 

PROVIDER: S-EPMC9023604 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature