Characterization of Paeniclostridium sordellii Metalloproteinase-1 in vitro and in an experimental model of infection.

ABSTRACT:

Objective

Paeniclostridium sordellii is a pathogen that causes rapidly fatal infections characterized by severe edema, extreme leukemoid reaction and lack of an innate immune response. We recently identified a metalloproteinase of P. sordellii-1 (Mcs1) that cleaves human vascular cell adhesion molecule 1, an adhesion molecule important to hematopoietic precursor retention and leukocyte diapedesis. In the current study, we further characterize Mcs1 activity and investigate its role in pathogenesis.

Methods

Mcs1 peptide cleavage sequence and activity conditions were identified using a semi-quantitative fluorescence-based reporter assay. Additional host targets for Mcs1 protease activity were tested and confirmed by gel electrophoreses and western blots. Finally, Mcs1 knock out

SUBMITTER: French JM 

PROVIDER: S-EPMC9023605 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature