Human stem cell-derived thymic epithelial cells enhance human T-cell development in a xenogeneic thymus.

ABSTRACT:

Background

Generation of thymic tissue from pluripotent stem cells would provide therapies for acquired and congenital thymic insufficiency states.

Objectives

This study aimed to generate human thymic epithelial progenitors from human embryonic stem cells (hES-TEPs) and to assess their thymopoietic function in vivo.

Methods

This study differentiated hES-TEPs by mimicking developmental queues with FGF8, retinoic acid, SHH, Noggin, and BMP4. Their function was assessed in reaggregate cellular grafts under the kidney capsule and in hybrid thymi by incorporating them into swine thymus (SwTHY) grafts implanted under the kidney capsules of immunodeficient mice that received human hematopoietic stem and progenitor cells (hHSPCs) intravenously.

Results

Cultured hES-TEP

SUBMITTER: Gras-Pena R 

PROVIDER: S-EPMC9023620 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature