The T-Type Calcium Channel Cav3.2 in Somatostatin Interneurons in Spinal Dorsal Horn Participates in Mechanosensation and Mechanical Allodynia in Mice.

ABSTRACT: Somatostatin-positive (SOM⁺) neurons have been proposed as one of the key populations of excitatory interneurons in the spinal dorsal horn involved in mechanical pain. However, the molecular mechanism for their role in pain modulation remains unknown. Here, we showed that the T-type calcium channel Cav3.2 was highly expressed in spinal SOM⁺ interneurons. Colocalization of Cacna1h (which codes for Cav3.2) and SOM ^tdTomato was observed in the in situ hybridization studies. Fluorescence-activated cell sorting of SOM ^tdTomato cells in spinal dorsal horn also proved a high expression of Cacna1h in SOM⁺ neurons. Behaviorally, virus-mediated knockdown of Cacna1h in spinal SOM⁺ neurons reduc

SUBMITTER: Zhi YR 

PROVIDER: S-EPMC9024096 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature