Evidence for Seed Transmission of Xylella fastidiosa in Pecan (Carya illinoinensis).

ABSTRACT: Pecan bacterial leaf scorch, caused by Xylella fastidiosa subsp. multiplex, is an economically significant disease of pecan with known detrimental effects on the yield of susceptible cultivars. In this study, endosperm was harvested from developing pecan seeds, and direct qPCR and sequencing were used to detect and confirm the presence of X. fastidiosa. DNA was isolated from mature seeds originating from seven trees, revealing a positivity rate up to 90%, and transmission of X. fastidiosa from infected seed to the germinated seedlings was found to be over 80%. Further epidemiological analyses were performed to determine where X. fastidiosa localizes in mature seed and seedlings. The highest concentrations of X. fastidiosa DNA were found in the hilu

SUBMITTER: Cervantes K 

PROVIDER: S-EPMC9024359 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature