Molecular Recognition of Proteins through Quantitative Force Maps at Single Molecule Level.

ABSTRACT: Intermittent jumping force is an operational atomic-force microscopy mode that produces simultaneous topography and tip-sample maximum-adhesion images based on force spectroscopy. In this work, the operation conditions have been implemented scanning in a repulsive regime and applying very low forces, thus avoiding unspecific tip-sample forces. Remarkably, adhesion images give only specific rupture events, becoming qualitative and quantitative molecular recognition maps obtained at reasonably fast rates, which is a great advantage compared to the force-volume modes. This procedure has been used to go further in discriminating between two similar protein molecules, avidin and streptavidin, in hybrid samples. The adhesion maps generated scanning with biotinylated probes showed features identi

SUBMITTER: Marcuello C 

PROVIDER: S-EPMC9024611 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature