The Effect of Amino Acids on Production of SCFA and bCFA by Members of the Porcine Colonic Microbiota.

ABSTRACT: Functional amino acids supplementation to farm animals is considered to not only be beneficial by regulating intestinal barrier, oxidative stress, and immunity, but potentially also by impacting the gut microbiota. The impact of amino acids on a piglet-derived colonic microbiota was evaluated using a 48-h in vitro batch incubation strategy. The combination of 16S rRNA gene profiling with flow cytometry demonstrated that specific microbial taxa were involved in the fermentation of each of the amino acids resulting in the production of specific metabolites. Branched chain amino acids (leucine, isoleucine, valine) strongly increased branched-chain fatty acids (+23.0 mM) and valerate levels (+3.0 mM), coincided with a marked increase of Peptostreptococcaceae. Further, glutamine and glut

SUBMITTER: Van den Abbeele P 

PROVIDER: S-EPMC9025589 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature