One-Week Scutellar Somatic Embryogenesis in the Monocot Brachypodium distachyon.

ABSTRACT: Plant somatic embryogenesis (SE) is a natural process of vegetative propagation. It can be induced in tissue cultures to investigate developmental transitions, to create transgenic or edited lines, or to multiply valuable crops. We studied the induction of SE in the scutellum of monocots with Brachypodium distachyon as a model system. Towards the in-depth analysis of SE initiation, we determined the earliest stages at which somatic scutellar cells acquired an embryogenic fate, then switched to a morphogenetic mode in a regeneration sequence involving treatments with exogenous hormones: first an auxin (2,4-D) then a cytokinin (kinetin). Our observations indicated that secondary somatic embryos could already develop in the proliferative calli derived from immature zygotic embryo tissu

SUBMITTER: Wehbi H 

PROVIDER: S-EPMC9025947 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature