Practical Considerations and Guidelines for Spectral Referencing for Fluorine NMR Ligand Screening.

ABSTRACT: Fluorine (¹⁹F) NMR strategies are increasingly being employed for evaluating ligand binding to macromolecules, among many other uses. ¹⁹F NMR offers many advantages as a result of its sensitive spin 1/2 nucleus, 100% natural abundance, and wide chemical shift range. Moreover, because of its absence from biological samples, one can directly monitor ligand binding without background interference from the macromolecule. Therefore, all these aforementioned features make it an attractive approach for screening compounds. However, the detection of ligand binding, especially those with weak affinities, can require interpretations of minor changes in chemical shifts. Thus, chemical shift referencing is critical for accurate measurements and interpretations. Unfortunately, one

SUBMITTER: Ayotte Y 

PROVIDER: S-EPMC9026065 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature