Molecular and cellular dynamics of early embryonic cell divisions in Volvox carteri.

ABSTRACT: Cell division is fundamental to all organisms and the green alga used here exhibits both key animal and plant functions. Specifically, we analyzed the molecular and cellular dynamics of early embryonic divisions of the multicellular green alga Volvox carteri (Chlamydomonadales). Relevant proteins related to mitosis and cytokinesis were identified in silico, the corresponding genes were cloned, fused to yfp, and stably expressed in Volvox, and the tagged proteins were studied by live-cell imaging. We reveal rearrangements of the microtubule cytoskeleton during centrosome separation, spindle formation, establishment of the phycoplast, and generation of previously unknown structures. The centrosomes participate in initiation of spindle formation and determination of spindle orientation. Altho

SUBMITTER: von der Heyde EL 

PROVIDER: S-EPMC9026201 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature